Superman
First: Kirk Alyn, the film serial Superman (1948)
Latest: Henry Cavill, Justice League (2017)
Batman
First: Lewis Wilson, film serial Batman (1943)
Latest: Ben Affleck, Justice League (2017)
Wonder Woman
First: Cathy Lee Crosby, TV movie Wonder Woman (1974)
Latest: Gal Gadot, Justice League (2017)
The Flash
First: John Wesley Shipp, TV series The Flash (1990)
Latest: Ezra Miller, Justice League (2017)
Green Lantern
First: Howard Murphy, TV special Legend of the Superheroes (1979)
Latest: Ryan Reynolds, Green Lantern (2011)
Catwoman
First: Julie Newmar, TV series Batman (1966)
Latest: Camren Bicondova, TV series Gotham (2014)
Supergirl
First: Helen Slater, Supergirl (1984)
Latest: Melissa Benoist, TV series Supergirl (2015)
Joker
First: Cesar Romero, TV series Batman (1966)
Latest: Joaquin Phoenix, Joker (2019)
Harley Quinn
First: Mia Sara, TV series Birds of Prey (2002)
Latest: Margot Robbie, Suicide Squad (2016)
Storm
First: Halle Berry, X-Men (2000)
Latest: Alexandra Shipp, Dark Phoenix (2019)
Wolverine
First: Hugh Jackman, X-Men (2000)
Latest: Hugh Jackman, Logan (2017)
Iron Man
First: Robert Downey Jr., Iron Man (2008)
Latest: Robert Downey Jr., Avengers: Endgame (2019)
Captain America
First: Reb Brown, TV movie Captain America (1979)
Latest: Chris Evans, Avengers: Endgame (2019)
Hulk
First: Lou Ferrigno, TV series The Incredible Hulk (1978)
Latest: Mark Ruffalo, Avengers: Endgame (2019)
Spider-Man
First: Nicholas Hammond, TV movie Spider-Man (1977)
Latest: Tom Holland, Spider-Man: Far From Home (2019)
Thor
First: Eric Kramer, TV film The Incredible Hulk Returns (1988)
Latest: Chris Hemsworth, Avengers: Endgame (2019)
Daredevil
First: Rex Smith, TV film The Trial of the Incredible Hulk (1989)
Latest: Charlie Cox, TV series Daredevil (2015)
Nick Fury
First: David Hasselhoff, TV movie Nick Fury: Agent of S.H.I.E.L.D. (1998)
Latest: Samuel L. Jackson, Spider-Man: Far From Home (2019)
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plus the tv series "the flash" is on now. so there a later one